Silica-Coated Superparamagnetic Iron oxide Nanoparticles Are More Durable for Labeling Mesenchymal Stem Cells Than Poly(Ethylene Glycol)-Coated Counterparts: Pilot in-vivo Assay Results
نویسندگان
چکیده
Y-X. Wang, K. C. Leung, T. Quercy-Jouvet, H. H. Wang, C. P. Chak, S. Lin, D. F. Wang, D. W. Au, P. C. Leung, and K. P. Fung Department of Imaging and Interventional Radiology, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, Center of Novel Functional Molecules, Department of Chemistry, The Chinese University of Hong Kong, Hong Kong, Department of Imaging and Interventional Radiology, The Chinese University of Hong Kong, Hong Kong, 4Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong, Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong
منابع مشابه
Evaluation of umbilical cord mesenchymal stem cell labeling with superparamagnetic iron oxide nanoparticles coated with dextran and complexed with Poly-L-lysine.
OBJECTIVE The objective of this study was to evaluate the effect of the labeling of umbilical cord vein derived mesenchymal stem cells with superparamagnetic iron oxide nanoparticles coated with dextran and complexed to a non-viral transfector agent transfector poly-L-lysine. METHODS The labeling of mesenchymal stem cells was performed using the superparamagnetic iron oxide nanoparticles/dext...
متن کاملIn vitro Labeling of Neural Stem Cells with Poly-L-Lysine Coated Super Paramagnetic Nanoparticles for Green Fluorescent Protein Transfection
Background: The magnetic nanoparticle-based transfection method is a relatively new technique for delivery of functional genes to target tissues. We aimed to evaluate the transfection efficiency of rat neural stem cell (NSC) using poly-L-lysine hydrobromide (PLL)-coated super paramagnetic iron oxide nanoparticles (SPION). Methods: The SPION was prepared and coated with PLL as transfection agent...
متن کاملEfficacy and Durability in Direct Labeling of Mesenchymal Stem Cells Using Ultrasmall Superparamagnetic Iron Oxide Nanoparticles with Organosilica, Dextran, and PEG Coatings
We herein report a comparative study of mesenchymal stem cell (MSC) labeling using spherical superparamagnetic iron oxide (SPIO) nanoparticles containing different coatings, namely, organosilica, dextran, and poly(ethylene glycol) (PEG). These nanomaterials possess a similar SPIO core size of 6-7 nm. Together with their coatings, the overall sizes are 10-15 nm for all SPIO@SiO₂, SPIO@dextran, a...
متن کاملThe effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors
INTRODUCTION Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated γ-Fe2O3) and studied their effect on proli...
متن کاملThe effects of grafted mesenchymal stem cells labeled with iron oxide or cobalt-zinc-iron nanoparticles on the biological macromolecules of rat brain tissue extracts
INTRODUCTION Rat mesenchymal stem cells (rMSCs) labeled with 1) poly-l-lysine-coated superparamagnetic iron oxide nanoparticles or 2) silica-coated cobalt-zinc-iron nanoparticles were implanted into the left brain hemisphere of rats, to assess their effects on the levels of oxidative damage to biological macromolecules in brain tissue. METHODS Controls were implanted with unlabeled rMSCs. Ani...
متن کامل